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Cloning and Expression
Enzymes
AvrII
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AvrII
AvrII from New England Biolabs
Be the first to write a review!
Citations:
(25)
Supplier Page
Supplier Page from
New England Biolabs for
AvrII
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Product Specs
Company Info
Citations
Reviews
Product Specs
Item
AvrII
Company
New England Biolabs
Price
Pricing Info
Supplier Page
View Company Product Page
Catalog Number
R0174L
Concentration
4 u/µL
Quantity
500 units
Applications
DNA Manipulation
Brand/Trademark
New England Biolabs Inc (NEB)
Type
Restriction Enzyme
Temperature
-20 C
Sequence
C/CTAGG
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New England Biolabs
240 County Road
Ipswich
,
Massachusetts
1938
United States
Phone
: 800-632-5227
800-632-7799
978-921-1350
Company Profile
Website:
http://www.neb.com
Citations (25)
(1)
Changes in searching behaviour of CSL transcription complexes in Notch active conditions
Life Sci Alliance
December 14, 2023
Sarah Baloul, Charalambos Roussos, Maria Gomez-Lamarca, Leila Muresan, Sarah Bray
TTTCTAGAGACAGCCGGAAATCTCGAGCGTGG for Hairless::Halo. Plasmids and HaloTag
PCR
products were digested using
AvrII
(
R0174S
;
New England Biolabs
) and
MluI
(
R0198S
;
NEB
) for Su(H)::Halo, or
BglII
(
R0144L
;
NEB
) and
XbaI
(
R0145S
;
(2)
S RNA Intergenic Deletions Drive Viral Interference during Arenavirus Infections
bioRxiv
November 7, 2023
Matthew Hackbart, Carolina B. López
include the full-length GPC and an mCherry gene replacing the NP gene by digestion with
AvrII
(
NEB
,
R0174S
), then insertion gene fragments with NEBuilder HIFI DNA Assembly Mix (
NEB
,
E5520S
). For
transfection
(3)
The Effect of Select SARS-CoV-2 N-Linked Glycan and Variant of Concern Spike Protein Mutations on C-Type Lectin-Receptor-Mediated Infection
Viruses
September 9, 2023
Arjan Bains, Wenyan Guan, Patricia J. LiWang
HindIII-HF
(
New England Biolabs
Cat #
R3104S
, Ipswich, MA, USA) and
AvrII
(
New England Biolabs
Cat #
R0174S
, Ipswich, MA, USA). To verify the presence of asparagine-to-glutamine spike mutations, the vectors were
(4)
AP profiling resolves co-translational folding pathway and chaperone interactions in vivo
bioRxiv
September 8, 2023
Xiuqi Chen, Christian M. Kaiser
SacI
(
NEB
,
R3156S
) to prevent exoIII digestion on the arrest peptide side and then with
AvrII
(
NEB
,
R0174S
) to allow for exoIII digestion towards the candidate protein. Linearized dsDNA was purified and quantified
(5)
How chromosomal translocations arise to cause cancer: Gene proximity, trans-splicing, and DNA end joining
iScience
June 16, 2023
Patrick Streb, Eric Kowarz, Tamara Benz, Jennifer Reis, Rolf Marschalek
hour at 37°C while shaking at 900 rpm. A total of 400 U restriction enzyme (for AFF1 and MLL:
AvrII
(
R0174L
,
NEB
); for KMT2B and CCND3:
HindIII
(R3104,
NEB
) was added and incubated overnight at 37°C while shaking
(6)
Structure, sequon recognition and mechanism of tryptophan C-mannosyltransferase
Nature Chemical Biology
May 1, 2023
Joël S. Bloch, Alan John, Runyu Mao, Somnath Mukherjee, Jérémy Boilevin, Rossitza N. Irobalieva, Tamis Darbre, Nichollas E. Scott, Jean-Louis Reymond, Anthony A. Kossiakoff,
et al.
into Pichia pastoris For each mutant, 15 µg of plasmid DNA was linearized overnight using
AvrII
(
NEB
,
R0174S
) then purified by ethanol precipitation. This DNA was transformed into electrocompetent GS115 P. pastoris
(7)
Systematically attenuating DNA targeting enables CRISPR-driven editing in bacteria
Nature Communications
February 8, 2023
Daphne Collias, Elena Vialetto, Jiaqi Yu, Khoa Co, Éva d. H. Almási, Ann-Sophie Rüttiger, Tatjana Achmedov, Till Strowig, Chase L. Beisel
the 1% agarose gel following the same conditions as described above for Cas9.
AvrII
(
NEB
, Cat. no.
R0174L
) was used to evaluate precise editing for the RT used in Fig. 1d.
BsgI
(
NEB
, Cat. no.
R0559L
) was used
(8)
Prediction of designer-recombinases for DNA editing with generative deep learning
Nature Communications
December 27, 2022
Lukas Theo Schmitt, Maciej Paszkowski-Rogacz, Florian Jug, Frank Buchholz
libraries were digested with the restriction enzymes
NdeI
and
AvrII
(
NEB
catalog number
R0111S
and
R0174S
), which will only cut in non-recombined plasmids. The digested DNA was then desalted with a MF-
Membrane
(9)
Protocol for production and expression of chimeric bovine-human monoclonal antibodies
STAR Protocols
December 16, 2022
Behnaz Heydarchi, Damian F.J. Purcell
Restriction enzyme,
New England Biolabs
,Cat# R3131LL
AvrII
Restriction enzyme,
New England Biolabs
,Cat#
R0174L
T4 DNA ligase
,
Thermo Fisher Scientific
,Cat#
EL0012
ExpiFectamine™ 293 Transfection Kit
,
Thermo Fisher Scientific
(10)
Broad and ultra-potent cross-clade neutralization of HIV-1 by a vaccine-induced CD4 binding site bovine antibody
Cell Reports Medicine
May 17, 2022
Behnaz Heydarchi et al.
Restriction enzyme,
New England Biolabs
,Cat# R3131LL
AvrII
Restriction enzyme,
New England Biolabs
,Cat#
R0174L
ExpiFectamine™ 293 Transfection Kit
,
Thermofisher
,Cat#
A14524
Protein G Agarose Fast Flow
,
Millipore
,Cat#
(11)
A Zika virus mutation enhances transmission potential and confers escape from protective dengue virus immunity
Cell Reports
April 12, 2022
Jose Angel Regla-Nava et al.
Triton X-100
,
Sigma
,Cat. #X100-100ML RNeasy Mini Kit,
Qiagen
,Cat. #
73404
AvrII
,(
New England BioLabs
),Cat. #
R0174S
SphI
,(
New England BioLabs
),Cat. #
R0182S
NaeI
,(
New England BioLabs
),Cat. #
R0190S
ClaI
,(
New England BioLabs
(12)
miR‐200a/b/‐429 downregulation is a candidate biomarker of tumor radioresistance and independent of hypoxia in locally advanced cervical cancer
Molecular Oncology
March 1, 2022
Anja Nilsen et al.
sequence with the restriction enzymes Nhe1 (
R3131S
;
New England Biolabs
, Ipswich, MA, USA) and
AvrII
(
R0174S
;
New England Biolabs
).
Lentivirus
was generated by co‐
transfection
of packaging plasmids and the pLenti
(13)
A bipartite element with allele-specific functions safeguards DNA methylation imprints at the Dlk1-Dio3 locus
Developmental cell
November 22, 2021
Aronson BE, Scourzic L, Shah V, Swanzey E, Kloetgen A, Polyzos A, Sinha A, Azziz A, Caspi I, Li J,
et al
.
(
NEB
, R0101) and Sall (NEB, R0138) and amplicon −3 was digested with
XhoI
(
NEB
, R0146) and
AvrII
(
NEB
,
R0174
). To insert four guides, the vector was digested with
XbaI
, amplicon −1 was digested with
XbaI
and
MfeI
,
(14)
Methylation of the tyrosine hydroxylase gene is dysregulated by cocaine dependence in the human striatum
iScience
October 22, 2021
Kathryn Vaillancourt, Gang G. Chen, Laura Fiori, Gilles Maussion, Volodymyr Yerko, Jean-François Théroux, Carl Ernst, Benoit Labonté, Erin Calipari, Eric J. Nestler,
et al.
identified in the topo TA cloning vector were released from the vector with a double digestion using
AvrII
(
R0174S
) and
SpeI
(
R0133S
-
New England Biolabs
) enzymes and gel
purification
(QIAquick Gel Extraction Kit,
Qiagen
,
(15)
Unraveling the hidden role of a uORF-encoded peptide as a kinase inhibitor of PKCs
Proceedings of the National Academy of Sciences
October 5, 2021
Divya Ram Jayaram et al.
(SI Appendix, Table S2). The resulted amplicon was then digested by
SacI
and
AvrII
(no.
R0156S
, no.
R0174S
,
New England Biolabs
, Inc. [NEB]) and cloned into the corresponding sites of the WT plasmid to generate
(16)
Development of a pan-neuronal genetic driver in Aedes aegypti mosquitoes
Cell Reports Methods
July 26, 2021
Zhilei Zhao, David Tian, Carolyn S. McBride
linearized with restriction enzymes
NsiI-HF
(
New England Biolabs
#
R3127S
) and
AvrII
(
New England Biolabs
#
R0174S
).[2]Syt1 left homology arm from ORL genomic DNA (NCBI: LOC5565901) with final seven Syt1 codons included
(17)
Precise genome engineering in
Drosophila
using prime editing
Proceedings of the National Academy of Sciences
January 14, 2021
Justin A. Bosch, Gabriel Birchak, Norbert Perrimon
132775) and gel purified. pNos-Cas9attB (56) was digested with
XbaI
/
AvrII
(
New England Biolabs
; R0145,
R0174
) to remove Cas9 sequences and the backbone fragment was gel purified. PE2 coding sequence was inserted
(18)
Comparison of Two Strategies to Generate Antigen-Specific Human Monoclonal Antibodies: Which Method to Choose for Which Purpose?
Frontiers in immunology
January 1, 2021
Ehlers AM, den Hartog Jager CF, Kardol-Hoefnagel T, Katsburg MMD, Knulst AC, Otten HG.
restriction enzyme,
R0553L
,
New England BioLabs
, Ipswich, Massachusetts, USAvrII restriction enzyme,
R0174L
,
New England BioLabs
, Ipswich, Massachusetts, UST4 Polynucleotide kinase,
M0201L
,
New England BioLabs
,
(19)
Integrative vectors for regulated expression of SARS-CoV-2 proteins implicated in RNA metabolism
Wellcome Open Research
November 3, 2020
Stefan Bresson, Nic Robertson, Emanuela Sani, Tomasz W Turowski, Vadim Shchepachev, Michaela Kompauerova, Christos Spanos, Aleksandra Helwak, David Tollervey
gene inserts, 1 µg of pUC containing the relevant gene was initially digested with
AvrII
(
NEB
, Cat No.
R0174S
) in the same reaction conditions followed by
purification
. The 5’ overhang, encoding the stop codon,
(20)
Generation of a Tumor-Specific Chemokine Gradient Using Oncolytic Vesicular Stomatitis Virus Encoding CXCL9
Molecular therapy oncolytics
March 27, 2020
Eckert EC, Nace RA, Tonne JM, Evgin L, Vile RG, Russell SJ.
Thermo Fisher Scientific
).
PCR
products were digested at 37°C for 16 h. Digestions consisted of
PCR
,
AvrII
(catalog number
R0174S
;
NEB
),
SbfI
(catalog number
R0642S
;
NEB
), and buffer B (
BB5
;
Thermo Fischer Scientific
). Primer sequences
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AvrII from New England Biolabs
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